To study the utility of ADEONASINE DEAMINASE activity in serum and body fluids for the diagnosis of T.B. The role of ADENOSINE DEAMINASE ENZYME Activity as diagnostic tool for tuberculosis pleural effusion & to differentiate tb pleural effusion from malignanat effusion.

In tuberculosis cellular immunity developes almost simultaneously with the delayed hyper sensitivity. The essential feature of d.h.s. is infiltration of non granular cells, including lymphocytes & marophages.

Currently T.B. is a global problem it is estimated that yearly about 10 millions people in the world are attacked with t.b. with 3 millions deaths.

Due to discovery of anti tuberculosis drugs the mortality & morbidity in the developed countries are decreased but it has not been eradicated from any country.

ADA catalysis the conversion of ammonia & inosine in purine metabolism. ADA is found widely in human tissues with its highest activity in lymphoid tissues. Among lymphocytes it is mainly associated in t.lymphocytes & studies have shown incrased ada levels in diseased character by t cell proliferation or activation.ADA has therefore been considered as a marker of cell mediated immunity.

Result of ada activity in serum of clinically proved extra pulmonary t.b. patients. The result shows that total positivity is 20% so there is a little role of ada activity in serum samples of extra pulmonary t.b. patients. Result of ada activity in pleural fluid clinically confirmed T.B.

The result shows that total ada activity in pleural fluid is 81.47% so it confirmed that ada activity is high in pleural effusion. The result shows that total positivity is 100% so there is a significant role of ada activity in pleural pus for diagnosis of T.B.

Result of ada activity in ascitic fluid clinically confirmed T.B.